General Estradiol (E2) ELISA Kit

Quantitative measurement of 17β-estradiol, the principal circulating estrogen, is supported by this competitive immunoassay from Assay Genie. The General Estradiol (E2) ELISA Kit targets the steroid hormone most commonly associated with ovarian function, menstrual cycle phase, follicular development, and broader hormonal regulation across mammalian systems.

Researchers investigating fertility outcomes, IVF protocols, polycystic ovary syndrome, menopausal transition, estrogen receptor signaling, or environmental endocrine disruption can apply the kit to serum-derived samples, plasma, tissue homogenates, cell lysates, and conditioned media from cultured cells. The competitive binding format is well suited to small-molecule analytes like estradiol, which are too small to be captured between two antibodies. Each microplate accommodates 96 individual determinations, and the colorimetric endpoint can be read on standard absorbance plate readers.

Each kit is intended for research use only and includes all reagents required for the stated number of assays. Refrigerated storage preserves conjugate and antibody activity through the indicated shelf life; certain components should not be frozen unless the supplied protocol explicitly permits this. Before drawing quantitative conclusions, researchers should follow the protocol's guidance on preparation of calibration standards, recommended dilution schemes for each sample type, and quality-control acceptance criteria.