General Dehydroepiandrosterone (DHEA) ELISA Kit

The Assay Genie General DHEA ELISA Kit applies a competitive immunoassay design in which DHEA present in the test specimen competes with a conjugated DHEA analogue for binding to a capture antibody immobilised on the microplate. Because signal intensity decreases as analyte concentration rises, this layout handles the relatively high circulating levels of DHEA, an adrenal-derived C19 steroid that acts as the principal biosynthetic precursor to androgens and oestrogens. The product is rated for Universal reactivity, which makes the protocol usable with serum and additional sample matrices across multiple species for cross-comparative endocrinology and adrenal function studies. Prepared calibrators and unknown samples should be aliquoted and kept refrigerated, with reconstituted material reserved to limit repeated freeze-thaw handling. The detection conjugate vial should be protected from light to preserve enzymatic activity, and all reagents are supplied for research-use-only in vitro laboratory work. Reagents are sufficient to complete the full microplate with standard curves and replicate unknowns.