General Cyclic Adenosine Monophosphate (cAMP) ELISA Kit

Quantify cyclic adenosine monophosphate (cAMP), a critical intracellular second messenger, with this competitive microplate immunoassay from Assay Genie. cAMP is generated by adenylyl cyclase following Gs-coupled GPCR activation and is degraded by phosphodiesterases, making it a central readout for receptor signaling, hormone action, and downstream PKA kinase pathways.

The kit is used to screen compounds for adenylyl cyclase modulation or phosphodiesterase inhibition, to profile GPCR ligand activity, and to measure cAMP dynamics across cell lysates, tissue homogenates, and cell culture supernatants subjected to pharmacological or genetic perturbations.

Delivered in a colorimetric 96-assay microplate format, the assay supports practical throughput for screening campaigns and pathway studies, enabling routine endpoint measurements and comparative analyses across treatment conditions. Intended for research use only, it serves academic and pharmaceutical laboratories investigating cell signaling, endocrinology, neuroscience, and cardiovascular biology.