General Arachidonic Acid (AA) ELISA Kit

The Competitive ELISA from Assay Genie quantifies free arachidonic acid (AA, 20:4n-6), a 20-carbon omega-6 polyunsaturated fatty acid liberated from membrane phospholipids during inflammation and serving as the primary substrate for prostaglandins, leukotrienes, and thromboxanes via the cyclooxygenase and lipoxygenase cascades. Provided in a 96 assays format, the assay supports both small pilot investigations and larger cohort screening in lipid signaling research.

Typical applications include profiling AA release in lipid metabolism studies, eicosanoid biosynthesis pathway analysis, and investigations of inflammatory mediator production across in vitro cell culture and in vivo models. Researchers apply this kit to diverse matrices — including serum samples drawn from metabolic disease cohorts, plasma collected in cardiovascular studies, and tissue homogenates prepared from animal models of neuroinflammation — making it a versatile tool for translational programmes.

Reagents are shipped lyophilized and require refrigerated storage to preserve antibody and enzyme conjugate activity over the stated shelf life. Standard microplate absorbance readers quantify arachidonic acid concentrations, enabling straightforward integration into existing laboratory workflows. The kit is intended for research use only.