General Androstenedione (ASD) ELISA Kit

From Assay Genie, this competitive immunoassay quantifies androstenedione (ASD), a 19-carbon steroid hormone that acts as a direct biochemical precursor to testosterone and estrone within the steroidogenesis pathway. Supplied in a 96 assays format with a chromogenic endpoint, the assay uses a competitive binding principle well matched to small steroid analytes that would not otherwise pair with two distinct capture reagents.

Validated across diverse starting materials — including tissue homogenates, cell lysates, cell culture supernatants, and standard clinical blood-derived samples — the protocol supports work with human specimens, animal-model tissues, and in vitro cell-based experiments. Because reactivity is described as universal, the same procedure can be transferred between species for comparative endocrinology projects, supporting translational research in reproductive biology, adrenal cortex function, polycystic ovary syndrome (PCOS), congenital adrenal hyperplasia, hirsutism, and disorders of androgen excess.

Each 96-assay plate kit provides the reagents needed to generate a standard curve and process the full plate, intended for laboratory research use only in studies investigating androgen biosynthesis and steroid hormone regulation.