General Androstenediol (AED) ELISA Kit

A competitive binding immunoassay from Assay Genie enables the quantitative determination of androstenediol (AED) in laboratory research samples. Androstenediol, also referred to as 5-androstenediol, functions as an intermediate metabolite within the steroid biosynthesis pathway running from pregnenolone through DHEA toward the active androgens testosterone and dihydrotestosterone. Quantifying this intermediate gives researchers a window into adrenal and gonadal steroid output that complements downstream testosterone measurements, supporting studies of enzymatic conversion, adrenal hyperactivity, and altered steroidogenesis in basic and clinical research settings.

The assay uses a competitive format in which free androstenediol in calibrators and samples competes with coated antigen for binding sites on a labelled antibody. After a wash step, the bound conjugate produces a measurable signal that is inversely related to analyte concentration, and the plate is read on a standard microplate reader. Researchers in endocrinology, reproductive biology, sports-medicine and anti-doping investigations, and clinical chemistry laboratories rely on this configuration for profiling adrenal-gonadal axis activity. The complete laboratory pack is supplied in a single box, with reagents prepared for the in vitro workflow described in the included protocol, and the product is intended for research use only.