General Adenosine Triphosphate (ATP) ELISA Kit

The Assay Genie General Adenosine Triphosphate (ATP) ELISA Kit provides a competitive immunoassay format for quantitative measurement of ATP, the universal cellular energy currency, across diverse biological matrices. The plate is pre-coated with a high-affinity capture antibody, and signal is generated through a colorimetric readout compatible with standard microplate readers. Each kit contains all reagents required to run a full microplate, including coated wells, standards, detection antibody, wash buffer, substrate, and stop solution, with reconstitution details outlined in the included protocol insert.

For sample handling, this assay accommodates several input types, including serum-derived samples, plasma-derived samples, tissue homogenates, and cell lysates, making it a practical tool for cell metabolism research, bioenergetics studies, mitochondrial function investigations, and drug screening workflows. The competitive format is particularly useful when measuring small molecules like ATP that cannot be bound by two-site capture methods. Researchers commonly include procedural controls such as spiked samples and standard curves in every run to verify linearity across the working range. Unopened kits should be refrigerated until first use, with reconstituted reagents aliquoted and frozen to limit freeze-thaw cycles. The microplate configuration supports multiple replicates per condition, and the assay is intended for research use only, not for diagnostic or clinical decision-making.