General 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit

Quantify oxidative DNA damage with this competitive immunoassay kit from Assay Genie, which measures 8-hydroxydeoxyguanosine (8-OHdG) in research specimens. 8-OHdG forms when reactive oxygen species attack guanine bases in DNA, and its accumulation serves as a widely cited indicator of oxidative stress in studies of carcinogenesis, neurodegeneration, cardiovascular pathology, and aging biology.

The kit is supplied as a complete reagent set, including pre-coated plate strips, calibrator standards, specific antibody, and chromogenic substrate, accommodating 96 tests per run in a microplate format. Researchers apply serum fractions, plasma fractions, urine, or cell culture supernatant, allowing the sample analyte to compete with a conjugated 8-OHdG reference for antibody binding sites. After substrate addition, absorbance readings enable quantitation against the included standard curve, with the colorimetric readout compatible with standard plate readers. The universal reactivity profile allows sample analysis across multiple species commonly used in laboratory research.

This reagent format supports basic research applications such as ROS-mediated damage investigations, environmental toxicology screening, and biomarker validation studies. Reagents should be refrigerated upon arrival and stored according to the package insert to preserve antibody activity, and the competitive binding chemistry provides tolerance for diverse sample matrices frequently encountered in translational research programs.