General 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) ELISA Kit

Assay Genie's competitive ELISA platform enables quantitative measurement of 8-epi-PGF2α (also termed 8-iso-PGF2α or 15-F2t-IsoP), a chemically stable F2-isoprostane generated by free-radical attack on membrane arachidonic acid and used as a clinical-research biomarker of systemic oxidative stress and lipid peroxidation. The microplate ships with 96 determination wells pre-coated with antigen, an HRP-conjugate, a lyophilised calibrator set spanning 15.63 to 1000 pg/mL, wash and TMB substrate buffers, and stop solution in clearly labelled vials. Published sensitivity for this Assay Genie design is 9.38 pg/mL, with no significant cross-reactivity or interference observed between 8-epi-PGF2α and related analogues. Each kit is supplied with a printed protocol insert describing a standard 2 h competitive incubation, conjugate preparation, and curve-fitting guidance, and the assay is intended for research use only. Recommended handling: equilibrate reagents to room temperature before use, aliquot the reconstituted standard after first thaw to limit repeated freeze-thaw cycles, and store kit components refrigerated; do not freeze the pre-coated plate. The format supports translational studies in atherosclerosis, neurodegeneration, diabetes complications, and pulmonary or renal ischemia-reperfusion injury, where lipid peroxidation is a primary endpoint.