General 5-Hydroxytryptamine (5-HT) ELISA Kit

Quantify serotonin levels with this competitive immunoassay from Assay Genie. 5-hydroxytryptamine (5-HT) is a critical monoamine neurotransmitter implicated in mood regulation, sleep architecture, appetite control, vascular tone, and gastrointestinal motility, making accurate measurement essential across neuroscience, psychopharmacology, and behavioral research.

The assay uses a competitive binding principle in which endogenous 5-HT in the specimen competes with enzyme-conjugated antigen for a limited number of antibody sites, and bound conjugate is measured via an enzymatic reaction producing a quantifiable optical signal. Universal reactivity validation allows researchers to apply the same kit across rodent, primate, and human-derived specimens, supporting translational studies without separate species-specific optimization.

The kit is configured to analyze serum-type and plasma-type specimens along with diverse experimental sample matrices commonly used in CNS tissue homogenates, cerebrospinal fluid work, and peripheral biomarker investigations. Reagents are supplied for 96 individual determinations, enabling moderate-throughput cohort screening or time-course sampling designs.

Each package includes a pre-coated microplate, lyophilized standards, detection antibody, substrate solution, stop reagent, and assay buffer. Store all components according to the enclosed protocol; protect from light and limit freeze-thaw cycles to preserve conjugate stability. For research use only — not intended for diagnostic procedures.

Researchers modeling depression, anxiety phenotypes, migraine mechanisms, or tryptophan metabolic pathways can integrate this kit with standard absorbance plate readers commonly available in laboratory settings.