General 25-Hydroxyvitamin D3 (HVD3) ELISA Kit

The General 25-Hydroxyvitamin D3 (HVD3) ELISA Kit from Assay Genie quantifies circulating 25-hydroxyvitamin D3 across research sample matrices using a competitive inhibition immunoassay workflow. The assay targets the principal circulating metabolite of vitamin D3 and supports investigations in bone metabolism, calcium homeostasis, endocrine function, and nutritional science. Pre-coated wells bind a labeled HVD3 conjugate that competes with endogenous analyte in the sample, producing a color signal inversely proportional to 25-hydroxyvitamin D3 concentration.

The kit supplies a microplate, calibrator standards, detection reagents, substrate, and stop solution configured for batch processing of up to 96 determinations. Reagents should be stored refrigerated to preserve antibody activity; protect from light guidance applies to substrate and conjugate vials, and the product is intended for research use only. The competitive format tolerates diverse biological sample types without extensive pre-processing, supporting routine screening and mechanistic studies of vitamin D biology in translational research.