GATA6 Transcription Factor Activity Assay

The GATA6 Transcription Factor Activity Assay from Assay Genie is a 96 Assays DNA-binding ELISA developed to quantify functional GATA6 binding in nuclear and cell lysates. The well plate is pre-coated with a double-stranded oligonucleotide containing the canonical GATA consensus motif (5'-WGATAR-3'), capturing active GATA6 from your sample. A GATA6-specific antibody then detects bound factor, with signal developed using an HRP substrate and read on a standard microplate reader. Validated for human, mouse, and rat samples, this kit supports research into endoderm specification, cardiac morphogenesis, pancreatic islet biology, and GATA6 dysregulation in cancer. The workflow involves nuclear extract preparation, plate incubation, washing, antibody detection, and absorbance measurement. Store kit components refrigerated; protect the HRP substrate from light and avoid freeze-thaw cycles of antibody reagents. For research use only.