GATA4 Transcription Factor Activity Assay

The GATA4 Transcription Factor Activity Assay from Assay Genie quantifies the DNA-binding activity of GATA4, a zinc-finger transcription factor central to cardiac morphogenesis, myocardial hypertrophy, and gonadal development. Researchers use this DNA-binding ELISA to measure active GATA4 in nuclear and cell lysates from human, mouse, and rat samples, supporting studies in cardiac biology, hepatocellular function, and sex determination pathways. The assay pairs a GATA4-specific capture antibody with an oligonucleotide containing the canonical GATA consensus (WGATAR) sequence immobilized on the well plate, allowing absorbance-based read-out of functional DNA binding rather than total protein expression. Typical applications include profiling transcription factor activation in stem cell-derived cardiomyocytes, monitoring GATA4 activity during cardiac differentiation, and screening compounds that modulate hypertrophic or developmental signaling. The kit is supplied as a 96 Assays package with the coated plate, nuclear extraction reagents, and detection antibodies for the workflow.