GATA3 Transcription Factor Activity Assay

The GATA3 Transcription Factor Activity Assay from Assay Genie quantifies the DNA-binding activity of activated GATA3 in nuclear and whole-cell lysates, distinguishing functionally engaged transcription factor from total cellular protein. Wells are pre-coated with a double-stranded oligonucleotide containing the consensus GATA-binding motif (WGATAR); nuclear extract is added, and DNA-bound GATA3 is detected with a GATA3-specific antibody in an absorbance-based readout.

Validated for human, mouse, and rat samples, this DNA-binding ELISA kit supports research into Th2 lymphocyte differentiation, mammary epithelial lineage commitment, and the role of GATA3 in luminal breast carcinoma and urothelial bladder cancer. The 96 Assays microplate format supports parallel interrogation of treatment conditions, dose responses, and time-course experiments.

Researchers studying signal-dependent transcription factor activation - for example following cytokine stimulation, hormonal challenge, or pathway inhibition - can pair this activity readout with Western blot or qPCR to confirm that changes in target gene expression track with changes in GATA3 DNA engagement. Typical workflow involves aliquoting nuclear extracts, addition to pre-coated wells, washes, and detection antibody incubation before chromogenic substrate development.