FOXO1A Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed for the precise quantification of FOXO1A protein levels in cell lysates, offering high sensitivity and specificity for molecular biology and drug development research. FOXO1A is a forkhead family transcription factor that regulates genes involved in cell cycle progression, apoptosis, and metabolism, and its dysregulation has been implicated in cancer, diabetes, and aging-related conditions.

The assay uses an indirect ELISA format in which FOXO1A is captured by target-specific primary antibodies, with HRP-conjugated secondary antibodies binding the Fc region of the primary antibody to catalyze a colorimetric reaction upon substrate addition. The kit is lysate-free and intended for use with adherent cells, enabling measurement of relative FOXO1A expression in cultured cells as well as screening for the effects of various treatments, inhibitors, or activators. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalization, and a Crystal Violet whole-cell staining step can be used to determine cell density for further data adjustment.

Reactivity is established for human, mouse, and rat samples, with a dynamic range of > 5000 Cells and detection at 450 nm. The kit is stored at 4°C with a stated stability of 6 Months.