FOXN2 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this colorimetric cell-based ELISA kit provides a sensitive, high-throughput method for the qualitative determination of FOXN2 protein levels in cultured cells using an indirect ELISA format. FOXN2-specific primary antibodies capture the target protein, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, enabling a colorimetric readout upon substrate addition. The kit is compatible with adherent cells, with an optional Poly-L-Lysine pre-coating step for suspension or loosely attached cells, allowing measurement of relative FOXN2 expression and screening of the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators.

Detection is read at 450 nm, with a dynamic range of > 5000 cells. The kit includes an anti-GAPDH monoclonal antibody that serves as an internal positive control for normalizing target absorbance values, and supports an optional Crystal Violet whole-cell staining step to determine cell density and adjust for plating differences. The protocol seeds 20,000 adherent cells per well in 200 µL of culture medium in the provided 96-well microplate, which is sterile and treated for cell culture.

FOXN2 (Forkhead box protein N2, UniProt P32314) is a transcription factor involved in regulating cellular processes including cell differentiation and proliferation, with dysregulation linked to numerous diseases. The kit is supplied in a 96-well microplate format, stored at 4°C with a 6-month shelf life, and falls within the research area of Epigenetics and Nuclear Signaling.