FOXL1 Transcription Factor Activity Assay

The FOXL1 Transcription Factor Activity Assay from Assay Genie provides a quantitative readout of the DNA-binding activity of FOXL1, a forkhead-box transcription factor implicated in hepatic differentiation, gastric mucosal maintenance, and gastrointestinal development. The microplate is pre-coated with a double-stranded oligonucleotide harboring the consensus FOXL1 response element; FOXL1 present in nuclear or whole-cell lysates binds this sequence, and the captured complex is detected using a FOXL1-specific antibody, an HRP-conjugated secondary, and a chromogenic substrate. The kit has been validated with Human, Mouse, and Rat samples, enabling cross-species transcriptional regulation studies. Nuclear extracts should be aliquoted and stored at -80 degrees C to preserve binding activity across freeze-thaw cycles. The assay is supplied as a complete research-use-only kit for screening small molecules, cytokines, or gene-editing tools that modulate FOXL1-driven transcriptional programs.