FOXE3 Colorimetric Cell-Based ELISA

Manufactured by Assay Genie, this Cell-Based ELISA kit is designed for the qualitative detection of FOXE3 protein in cultured cells, supporting research into eye development and related disorders. FOXE3 is a forkhead-family transcription factor with a lens-specific role, and the kit enables researchers to monitor its expression profile under various treatment conditions.

The assay uses an indirect ELISA format in a 96-well microplate with colorimetric readout at 450 nm, and a dynamic range above 5000 cells. It is reactive with human, mouse, and rat samples and is intended for adherent cell cultures. Storage stability is rated at 4°C for 6 months, and the kit is positioned for Epigenetics and Nuclear Signaling research.

Capture relies on FOXE3-specific primary antibodies paired with HRP-conjugated secondary antibodies that catalyse a colour reaction upon substrate addition. Because the readout is qualitative, two normalisation methods are included: a monoclonal anti-GAPDH antibody as an internal positive control, and a Crystal Violet whole-cell staining step to account for cell density and plating differences.