FOS (Phospho-Thr232)Colorimetric Cell-Based ELISA Kit

A cell-based ELISA kit from Assay Genie is designed to detect phosphorylation of the FOS transcription factor at threonine 232 in cultured adherent cells. The assay uses an indirect, lysate-free ELISA format with a colorimetric readout at 450 nm, and is reactive with human, mouse, and rat samples with a dynamic range of > 5000 cells. FOS is a proto-oncogenic transcription factor of the bZIP family that dimerizes with JUN family proteins to form the AP-1 complex, regulating cell proliferation, differentiation, and transformation. Phosphorylation at threonine 232 is linked to several signaling pathways, including those involved in cellular stress response and signal transduction.

The kit is intended for measuring relative levels of phosphorylated FOS in cultured cells and for screening the effects of treatments, inhibitors (such as siRNA or chemicals), or activators. Because the assay is qualitative, two normalization strategies are provided: a monoclonal anti-GAPDH antibody serves as an internal positive control, and Crystal Violet whole-cell staining can be used to determine cell density for normalization. Database information includes Gene ID 2353 and UniProt ID P01100.