Fos (Phospho-Ser362)Colorimetric Cell-Based ELISA Kit

This cell-based colorimetric ELISA from Assay Genie is designed for the precise detection of phosphorylated Fos protein at serine 362, offering a dynamic range of > 5000 Cells. It provides a lysate-free, high-throughput workflow using an indirect ELISA format, in which a phospho-specific primary antibody captures the target and HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition, measured at 450 nm.

The kit is reactive with human, mouse, and rat samples and is intended for use with adherent cells. Researchers can use it to measure relative levels of phosphorylated Fos in cultured cells and to screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on Fos phosphorylation. Because the assay is qualitative, two normalization approaches are provided: a monoclonal anti-GAPDH antibody serves as an internal positive control for normalizing target absorbance, and Crystal Violet whole-cell staining may be used to determine cell density so that absorbance values can be adjusted for plating differences.

Phosphorylated Fos at serine 362 is implicated in regulating gene expression and cell growth, making the kit a useful tool for studying cellular signaling pathways and responses relevant to disease mechanisms.