FOS (Phospho-Ser32)Colorimetric Cell-Based ELISA Kit

Assay Genie's high-quality In-Cell ELISA Kit provides precise measurement of phosphorylated FOS at serine 32 in cell lysates and tissue samples. The kit offers a convenient, lysate-free, high-throughput and sensitive workflow for detecting FOS protein phosphorylation and expression profiles in cultured cells, and can be used for screening the effects of various treatments, inhibitors (e.g., siRNA or chemicals), or activators on FOS phosphorylation.

Designed as a Cell-Based assay reactive with Human, Mouse, and Rat samples, the kit uses adherent cells and a colorimetric readout at 450 nm, with a stated dynamic range of "> 5000 Cells". Qualitative determination is achieved through an indirect ELISA format: FOS (Phospho-Ser32) is captured by target-specific primary antibodies, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody and catalyze a colorimetric reaction upon substrate addition.

Because the assay is qualitative, two normalization approaches are included: a monoclonal antibody specific for human GAPDH serves as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining method can be used to determine cell density, allowing absorbance values to be analysed against cell amounts to adjust for plating differences.