FNDC5 DIY ELISA Kit

This FNDC5 ELISA kit from Assay Genie uses a color-based read-out and is built to quantify fibronectin type III domain-containing protein 5, the membrane-bound precursor that is proteolytically cleaved to release the myokine irisin into circulation. The reagents are supplied in a scalable DIY plate-coating format and are sufficient for researchers running their own immunoassays on human samples, with enough material to coat up to ten standard microplates. This supports both small pilot studies and larger cohort screening campaigns without batch-to-batch reagent variability.

FNDC5 and its cleavage product irisin are major targets in exercise physiology and energy metabolism research, where irisin has been reported to act on white adipose tissue and promote a brown adipocyte-like gene expression program. The kit is commonly paired with skeletal muscle cell cultures, exercised animal model samples, or clinical cohorts stratified by BMI, type 2 diabetes status, or physical activity, with irisin tracked as a circulating readout of myokine response. All components should be refrigerated upon arrival and stored refrigerated between uses to preserve capture antibody activity and standard curve stability across multiple plates.