FLT3 (Phospho-Tyr842)Colorimetric Cell-Based ELISA Kit

A Cell-Based ELISA Kit from Assay Genie designed for the qualitative detection of FLT3 phosphorylation at tyrosine 842 in cultured cells, supporting research on FLT3 receptor tyrosine kinase signalling and FLT3-driven cancers. The kit is reactive with Human and Mouse samples and uses a colorimetric readout at 450 nm via an indirect ELISA format, in which HRP-conjugated secondary antibodies bind primary antibodies specific for the phospho-Tyr842 site.

The assay is lysate-free and intended for use with adherent cells, enabling measurement of relative levels of phosphorylated FLT3 and screening of the effects of treatments, siRNA or chemical inhibitors, and activators on FLT3 phosphorylation. A monoclonal anti-GAPDH antibody is supplied as an internal positive control for normalisation, and a Crystal Violet whole-cell staining step can be used to relate absorbance values to cell density. The stated dynamic range is "> 5000 Cells".

FLT3 is a class III receptor tyrosine kinase of the PDGFR family (UniProt P36888; Gene ID 2322). Dysregulation of FLT3 signalling, including activating mutations, has been implicated in acute myeloid leukaemia (AML) and other haematological malignancies, making the Tyr842 phosphorylation site relevant to studies of FLT3 pathway activation. FLT3 inhibitors referenced in the literature include Sutent and PKC412.