Filamin A Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit is designed to detect Filamin A in cell lysates, tissue homogenates, and biological samples. It offers high sensitivity and specificity for studying this cytoskeletal protein, which plays a role in cell adhesion, migration, and signaling pathways. The kit is reactive with Human, Mouse, and Rat samples and uses a colorimetric detection method at 450 nm.

The assay uses an indirect ELISA format where Filamin A is captured by specific primary antibodies and HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalizing target absorbance values, and Crystal Violet staining may be used to determine cell density. The kit is supplied in a microplate format and is stable at 4°C for 6 Months.

Researchers can measure relative Filamin A amounts in cultured cells and screen the effects of treatments, inhibitors such as siRNA or chemicals, or activators. The procedure involves seeding adherent cells, fixation, quenching, blocking, primary antibody incubation, washing, and colorimetric measurement. The kit has a dynamic range of greater than 5000 cells.