FGFR3 Colorimetric Cell-Based ELISA Kit

Manufactured by Assay Genie, this cell-based ELISA kit is designed for the colorimetric detection of fibroblast growth factor receptor 3 (FGFR3) in cell samples. The assay is reactive with human, mouse, and rat samples and is suited to adherent cell preparations. Detection is carried out at 450 nm, with a stated dynamic range of more than 5,000 cells, in a 96-well microplate format.

The kit employs an indirect ELISA format in which FGFR3 is captured by target-specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction on substrate addition. Described as lysate-free and high-throughput, the assay can be used to measure the relative amount of FGFR3 in cultured cells and to screen the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on FGFR3.

A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control for normalising target absorbance values. Following HRP readout, a Crystal Violet whole-cell staining step can be applied to determine cell density, allowing absorbance to be normalised to cell amount and correcting for plating differences. The kit is stored at 4 °C with a stated stability of 6 months and is listed for research use in the area of cell death, supporting work in cell signalling, cancer biology, and developmental disorders where FGFR3 has been linked to skeletal dysplasias and cancers.