FGFR2 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit enables quantitative measurement of Fibroblast Growth Factor Receptor 2 (FGFR2) levels in cell samples, with reactivity validated for Human, Mouse, and Rat. The kit uses a Colorimetric detection method measured at 450 nm and is supplied in a 96-Well Microplate format. It is intended for use with adherent cells and covers a dynamic range of > 5000 Cells.

The assay is lysate-free, high throughput, and sensitive, employing an indirect ELISA format in which FGFR2-specific primary antibodies capture the target while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, allowing the conjugated HRP to catalyse a colour reaction upon substrate addition. It can be used to measure the relative amounts of FGFR2 in cultured cells and to screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on FGFR2. For normalisation, a monoclonal antibody specific for human GAPDH is included as an internal positive control, and a Crystal Violet whole-cell staining method is provided to determine cell density so that absorbance values can be normalised to cell amounts and plating differences adjusted.

The kit is stored at 4°C with a stability of 6 Months. It supports research into FGFR2, a receptor tyrosine kinase of the highly conserved FGFR family that binds fibroblast growth factor (FGF) and is involved in cell growth, differentiation, and tissue repair, with relevance to studies of cancer and developmental disorders. Database identifiers listed for the target include Gene ID: 2263 and UniProt ID: P21802.