FGFR1 (Phospho-Tyr154)Colorimetric Cell-Based ELISA Kit

This Cell-Based ELISA Kit from Assay Genie is designed for the qualitative detection of phosphorylated FGFR1 (fibroblast growth factor receptor 1) at tyrosine 154 in cell lysates. The assay is lysate-free, high-throughput, and sensitive, using an indirect ELISA format in which target-specific primary antibodies capture the phosphorylated protein and HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition.

The kit is intended for use with adherent cells and is reactive with Human, Mouse, and Rat samples. The dynamic range is > 5000 Cells, and detection is colorimetric, measured at 450 nm. Researchers can use it to measure the relative amounts of phosphorylated FGFR1 in cultured cells and to screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on FGFR1 phosphorylation.

Because the Cell-Based ELISA is qualitative, two normalization methods are provided: a monoclonal antibody specific for human GAPDH serves as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining method can be used to determine cell density, after which absorbance values can be normalized to cell amounts. Phosphorylation of FGFR1 at tyrosine 154 regulates its activity and signaling pathways involved in cell growth, differentiation, and survival, making the kit relevant for studies of cell signaling in cancer, developmental disorders, and cardiovascular diseases.