FES Colorimetric Cell-Based ELISA

A cell-based ELISA kit from Assay Genie designed to detect FES (Feline Sarcoma Oncogene) protein expression directly in cultured cells. FES is a non-receptor tyrosine kinase of the Fer family that contributes to normal hematopoiesis and is linked to the development of acute promyelocytic leukemia. The kit offers a convenient, lysate-free, high-throughput approach for measuring relative FES levels in adherent cells and for screening the effects that various treatments, inhibitors, or activators have on FES expression.

The assay uses an indirect ELISA format in which FES is captured by target-specific primary antibodies and detected via HRP-conjugated secondary antibodies that catalyze a colorimetric reaction measured at 450 nm. Reactivity covers human and mouse samples, and the dynamic range is reported as > 5000 cells. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, and Crystal Violet whole-cell staining can be used to determine cell density for further normalization.

The kit is supplied in a 96-well microplate format and is stored at 4°C with a stated stability of 6 months. The FES gene maps to chromosomal location 15q26.1 and encodes the tyrosine-protein kinase Fes/Fps with a molecular weight of 85,466 Da.