Feline TNF alpha DIY ELISA Kit

This DIY ELISA format from Assay Genie is supplied as capture antibody, detection antibody, recombinant standard, and buffers so the end user coats their own microplate, enabling flexible plate counts up to the rated capacity. The kit is built to quantitatively measure tumor necrosis factor alpha (TNF-alpha), a pro-inflammatory cytokine central to feline immunology, infectious-disease research, and veterinary vaccine studies.

• Detection range: 15.6 to 1000 pg/mL
• Sensitivity: typically below 5 pg/mL
• Sample volume: 100 uL per well
• Assay time: approximately 3.5 hours at room temperature
• Sample types: serum, plasma, and cell culture supernatants of feline origin
• Storage: short term at 4 degrees C; aliquot and freeze the standard at -20 degrees C and protect from light; limit freeze-thaw cycles
• Readout: colorimetric, measured on a standard microplate reader

Laboratories working on feline inflammatory responses, chronic kidney disease models, or feline immunodeficiency virus (FIV) research use this kit to quantify TNF-alpha secretion from stimulated peripheral blood mononuclear cells and tissue explants. All components are intended for research use only and are not validated for clinical diagnostic procedures.