FAK (Phospho-Tyr397)Fluorometric Cell-Based ELISA Kit

Assay Genie provides a fluorometric cell-based ELISA kit designed to monitor FAK (Phospho-Tyr397) protein phosphorylation and expression profile in cells. The kit uses a lysate-free, high-throughput format suitable for measuring the relative amounts of phosphorylated FAK in cultured cells and for screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on PTK2 phosphorylation.

Detection is achieved by an indirect ELISA approach in which target-specific primary antibodies are bound by Dye 1- and Dye 2-conjugated secondary antibodies, enabling fluorometric readout. The kit is reactive with human, mouse, and rat samples, with a dynamic range of > 5000 cells, and is supplied in a two 96-well plate format. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target RFU values, and an antibody against the non-phosphorylated counterpart of FAK (Phospho-Tyr397) is provided so that RFU values for non-phosphorylated FAK can be used to normalize the RFU value for phosphorylated FAK. Plates are read at Ex/Em 651/667 (Dye 1) and 495/521 (Dye 2), and the kit is stored at 4°C with a stated stability of 6 months.

Associated target identifiers listed for the product include NCBI Gene Symbol PTK2, Gene ID 5747, UniProt ID Q05397, OMIM # 600758, and Unigene # Hs.395482.