FADD (Phospho-Ser194)Colorimetric Cell-Based ELISA Kit

Designed by Assay Genie, this Colorimetric Cell-Based ELISA Kit enables the accurate quantification of FADD phosphorylation at serine 194 directly in cells. The target, FADD (Fas-associated death domain protein), is described as a key regulator of apoptosis that mediates cell death and survival pathways, and phosphorylation at this residue has been implicated in cell cycle regulation and immune response modulation.

The assay uses an indirect ELISA format in which FADD (Phospho-Ser194) is captured by phospho-specific primary antibodies and detected with HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, and Crystal Violet whole-cell staining is used to determine cell density so that results can be adjusted for plating differences. The kit is described as lysate-free and high throughput, validated for human samples and adherent cells, with a dynamic range of more than 5000 cells and detection read at 450 nm (Gene ID: 8772; UniProt ID: Q13158).