ETV6 Transcription Factor Activity Assay

The ETV6 Transcription Factor Activity Assay from Assay Genie quantitatively measures the DNA-binding activity of the ETV6 protein (also known as TEL), a member of the ETS family of transcription factors. The 96 Assays plate is pre-coated with a double-stranded oligonucleotide containing the consensus ETV6 binding sequence, capturing active ETV6 from nuclear and cell lysates prepared from human, mouse, or rat samples. Detection uses a colorimetric antibody-based readout, allowing researchers to monitor transcriptional activation, repression, and dysregulation linked to chromosomal translocations such as ETV6-RUNX1 and ETV6-NTRK3 frequently observed in leukemia studies. Each kit contains reagents sufficient for 96 Assays and supports chromatin biology research, hematopoiesis studies, and cancer signaling pathway investigations. Storage recommendation is typically at -20 degrees C for long-term stability; avoid repeated freeze-thaw cycles of the antibody components. The assay format supports a nuclear extract sample volume of approximately 10-20 ul per well, providing a sensitive method for profiling transcription factor activity in vitro without requiring electrophoresis or radiolabeled probes.