ETV4 Transcription Factor Activity Assay

ETV4, also known as PEA3 or E1AF, is a member of the ETS family of transcription factors that bind the 5'-GGAA-3' DNA consensus motif and regulate genes controlling cell migration, epithelial-mesenchymal transition, and metastatic progression in prostate, breast, and colorectal cancers. This Assay Genie DNA-binding ELISA kit captures functional ETV4 activity from nuclear and cell lysates via an immobilized target oligonucleotide, then uses a specific antibody and colorimetric readout to quantify DNA-bound transcription factor, providing a more physiologically relevant measurement than total protein expression. The assay cross-reacts with human, mouse, and rat samples, enabling cross-species studies in cancer biology, developmental signaling, and gene regulatory research. For long-term stability, store kit components at -20°C and avoid repeated freeze-thaw cycles of the antibodies and standards.