ETK (Phospho-Tyr566)Fluorometric Cell-Based ELISA Kit

A fluorometric cell-based phospho ELISA from Assay Genie enables qualitative monitoring of ETK (Phospho-Tyr566) phosphorylation and expression profile in cultured cells without the need for lysate preparation. The indirect ELISA format uses Dye-1 and Dye-2 conjugated secondary antibodies, allowing fluorometric readout at Ex/Em 651/667 and 495/521. A monoclonal GAPDH antibody is included as an internal positive control for normalizing target RFU values, and an antibody against the nonphosphorylated counterpart of ETK (Phospho-Tyr566) is provided for additional normalization of phosphorylated ETK signal.

The assay is suited for screening the influence that siRNA, chemicals, or other treatments and inhibitors have on ETK phosphorylation, with a dynamic range of > 5000 cells. Storage is at 4°C for 6 months. Listed reactivity covers human and mouse samples. The target corresponds to the BMX gene, with database identifiers Gene ID 660, UniProt ID P51813, OMIM #300101, and Unigene #Hs.495731.