ETK (Phospho-Tyr40)Colorimetric Cell-Based ELISA Kit

Designed for the accurate detection of phosphorylated tyrosine 40 levels in cellular extracts, this Colorimetric Cell-Based ELISA Kit from Assay Genie uses a lysate-free, high-throughput format to measure ETK protein phosphorylation and expression in cultured cells. The indirect ELISA format employs target-specific primary antibodies to capture the phospho-protein, with HRP-conjugated secondary antibodies catalyzing a colorimetric reaction read at 450 nm, providing a qualitative readout suitable for studying cell signaling and screening the effects of various treatments, inhibitors (e.g. siRNA or chemicals) or activators on ETK phosphorylation.

Reactive with human and mouse samples and validated for adherent cells, the assay covers a dynamic range of > 5000 cells and supports normalization through two built-in controls: a monoclonal GAPDH antibody supplied as an internal positive control, and a Crystal Violet whole-cell staining step for adjusting absorbance values to cell density. The target corresponds to the BMX gene (Gene ID 660; UniProt P51813), which encodes a non-receptor tyrosine kinase of the Tec family implicated in several signal transduction pathways, including the Stat pathway, and in the differentiation and tumorigenicity of various cancer cell types.