Estrogen Receptor-alpha (Phospho-Tyr537)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit is designed to detect and quantify phosphorylated Estrogen Receptor-alpha at Tyr537 in cell lysates, with a stated dynamic range of > 5000 Cells. It is reactive with human and mouse samples and is intended for use with adherent cells.

The kit employs an indirect ELISA format and is lysate-free, providing a high-throughput, sensitive approach for the qualitative measurement of Estrogen Receptor-alpha (Phospho-Tyr537) in cultured cells. Detection relies on HRP-conjugated secondary antibodies that catalyse a colorimetric reaction measured at 450 nm. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining step can be used to determine cell density, allowing absorbance readings to be adjusted for plating differences.

Researchers can use the assay to measure relative phosphorylation levels in cells and to screen the effects of various treatments, inhibitors (such as siRNA or chemicals) and activators on Estrogen Receptor-alpha phosphorylation. The target is associated with Gene ID 2099, UniProt ID P03372 and the gene symbol ESR1, and phosphorylation at Tyr537 is linked to hormone resistance in breast cancer, making the kit relevant to studies of estrogen signalling and a noted target for therapeutic intervention.