Estrogen Receptor-alpha (Phospho-Ser118)Colorimetric Cell-Based ELISA Kit

A colorimetric cell-based ELISA kit from Assay Genie is designed for the detection of Estrogen Receptor-alpha phosphorylated at Ser118 in adherent cells. The assay uses an indirect ELISA format in which the phospho-target is captured by phospho-specific primary antibodies and detected with HRP-conjugated secondary antibodies that catalyse a colorimetric reaction read at 450 nm. It can be used to measure relative phosphorylation levels of Estrogen Receptor-alpha in cultured cells and to screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on phosphorylation. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising target absorbance values, and Crystal Violet whole-cell staining may be used to determine cell density after the colorimetric measurement.

The kit is reactive with Human and Mouse samples and has a dynamic range of > 5000 Cells. The associated target gene is ESR1 (Gene ID: 2099, UniProt ID: P03372), and the target is classified under a phospho sub type. According to the manufacturer's UniProt reference, Estrogen Receptor-alpha is described as a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.