ERK8 Colorimetric Cell-Based ELISA Kit

From Assay Genie, the ERK8 Colorimetric Cell-Based ELISA Kit is designed for the precise measurement of ERK8 (Extracellular Signal-Regulated Kinase 8) activity in cell lysates and signalling pathway studies, and is suitable for adherent cell samples. The assay uses a colorimetric detection method at 450 nm and shows reactivity with human and mouse samples.

This lysate-free, high-throughput cell-based ELISA detects ERK8 protein expression profiles in cultured cells, with a dynamic range of > 5000 Cells. It can be used to measure relative amounts of ERK8 in cultured cells and to screen for the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on ERK8. Storage is at 4 °C with a stated stability of 6 months.

Qualitative determination of ERK8 concentration is achieved through an indirect ELISA format. ERK8 is captured by ERK8-specific primary antibodies, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. The HRP enzyme then catalyses a colourimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining method can be used to determine cell density and adjust for plating differences. ERK8 plays a key role in cell signalling pathways, regulating cellular processes such as cell growth, differentiation, and survival, and its dysregulation has been implicated in cancer, inflammatory disorders, and neurodegenerative diseases.