ERK3 (Phospho-Ser189)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this colorimetric cell-based ELISA kit is designed for the precise detection of phosphorylated ERK3 (Phospho-Ser189) protein levels in cell lysates from adherent cells. The assay uses a lysate-free, high-throughput workflow in an indirect ELISA format: target-specific primary antibodies capture ERK3 phosphorylated at Serine 189, and HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition, with absorbance read at 450 nm. Reactivity is established for human, mouse, and rat samples, and the dynamic range is > 5000 cells.

The kit supports qualitative measurement of relative phosphorylated ERK3 levels in cultured cells and is suitable for screening the effects of treatments, siRNA, chemicals, or other activators on ERK3 phosphorylation. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalizing target absorbance values, and Crystal Violet whole-cell staining is provided to determine cell density and correct for plating differences. ERK3 (gene symbol MAPK6, UniProt Q16659) is a member of the MAPK family with a reported role in cell growth and differentiation signaling, and the kit is targeted to the cell cycle research area.

Supplied components include anti-phospho and anti-total primary antibodies, HRP-conjugated anti-rabbit and anti-mouse secondary antibodies, wash buffer, blocking buffer, quenching buffer, primary antibody diluent, substrate, stop solution, SDS solution, Crystal Violet solution, and adhesive plate seals.