ERK3 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit is designed for the precise measurement of ERK3 levels in cell lysates, tissue homogenates and other biological samples. With high sensitivity and specificity, the kit is reported to provide accurate and reproducible results suited to a range of research applications.

ERK3, also known as MAPK6, is described as a critical signalling protein involved in cell growth, survival and differentiation, with dysregulation linked to cancer and inflammatory disorders. The assay is a convenient, lysate-free, high-throughput format that detects the ERK3 protein expression profile in cells, making it suitable for measuring relative ERK3 levels in cultured cells and for screening the effects of treatments, inhibitors (such as siRNA or chemicals) or activators.

Qualitative determination is achieved through an indirect ELISA approach in which ERK3 is captured by ERK3-specific primary antibodies and HRP-conjugated secondary antibodies bind the Fc region of those primaries, catalysing a colour change upon substrate addition. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalising target absorbance, and Crystal Violet whole-cell staining may be used to determine cell density after the colourimetric read-out. The kit is reactive with human, mouse and rat samples and is intended for use with adherent cells. Detection is colourimetric at 450 nm, with a dynamic range above 5000 cells. The kit is stored at 4 °C with a stated stability of 6 months. The target protein has a reported molecular weight of 82,681 Da (UniProt ID Q16659; Gene ID 5597).