Equine Interleukin 4 (IL-4) ELISA Kit

The Assay Genie Equine Interleukin 4 (IL-4) ELISA Kit enables precise quantification of IL-4 in equine research samples, supporting studies of Th2-mediated immunity, allergic inflammation, and parasite response in horses. The standard curve spans 15.6 to 1000 pg/mL with a minimum detectable dose of approximately 7.8 pg/mL. Each well requires 50 µL of sample. The microplate arrives pre-coated with capture antibodies, and the workflow uses a biotin-streptavidin-HRP detection system with TMB substrate. Reagents should be refrigerated at 4°C; the kit is intended for research use only and is not validated for diagnostic procedures. With intra-assay and inter-assay precision typically below 10% CV, this kit supports reproducible cytokine profiling in cell culture supernatants, tissue homogenates, and lysate preparations.