Equine Interleukin 1 Alpha (IL-1a) ELISA Kit

Equine IL-1α is a key pro-inflammatory cytokine released by activated macrophages and epithelial cells during infection, tissue injury, and stress responses. Assay Genie's Equine Interleukin 1 Alpha ELISA Kit provides a quantitative immunoassay for measuring native IL-1α protein across equine research applications including laminitis, joint inflammation, endotoxemia, and reproductive immunology studies.

The microplate is pre-coated with a capture antibody specific to equine IL-1α. Researchers run a standard curve alongside unknown samples using a sample volume of 100 uL per well. Reagents are supplied ready-to-use, including biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate, and stop solution. Readout produces data proportional to IL-1α concentration in serum, plasma, tissue homogenates, cell lysates, and cell culture supernatants.

Allow all kit components to equilibrate to room temperature before use, and avoid repeated freeze-thaw cycles of prepared samples. This research-use-only kit from Assay Genie is intended for laboratory studies of equine immune signaling and is not validated for diagnostic or veterinary clinical use.