Equine Eosinophil Chemotactic Factor (ECF) ELISA Kit

For equine immunology research, this Assay Genie immunoassay enables quantitative measurement of Eosinophil Chemotactic Factor in horse-derived samples, supporting investigations of allergic airway inflammation, parasitic helminth responses, and eosinophil recruitment mechanisms in horses. The kit supplies all reagents needed to perform 96 individual determinations on a microplate, including pre-coated wells, lyophilized reference standards in sealed vials, biotinylated detection reagent, enzyme conjugate working solution, chromogenic substrate, and stop reagent. Sealed components should be stored refrigerated between uses to preserve reagent activity throughout the kit's shelf life.

Colorimetric endpoint absorbance is read on a standard plate reader, with the protocol accommodating blood-derived fractions and tissue-derived homogenates from equine subjects. Veterinary respiratory research groups, equine asthma investigators, and academic teams studying chemokine signaling in the horse apply this kit to quantify ECF concentrations across varied specimen types. Common applications include recurrent airway obstruction models, strongyle parasite studies, and allergic dermatitis research, where eosinophil chemotaxis measurements inform understanding of type-2 immune responses in equids.