Equine Angiogenin (ANG) ELISA Kit

The Assay Genie Equine Angiogenin (ANG) ELISA Kit measures endogenous angiogenin, a 14 kDa secreted ribonuclease implicated in angiogenesis, vascular repair, and placental development in horses. Liquid equine samples and prepared tissue homogenates are processed in a microplate pre-coated with a species-matched capture antibody, and the assay uses a biotinylated detector with a streptavidin-HRP conjugate to generate a signal proportional to angiogenin concentration. Results are interpolated against a reconstituted lyophilized standard curve run on each plate. Each kit includes sufficient reagents for one full plate plus reference standards, biotin conjugate, enzyme conjugate, wash buffer, chromogenic substrate, and stop solution. Researchers in equine sports medicine, neonatal care, and reproductive biology apply this quantitative angiogenin ELISA to monitor angiogenic activity during tendon recovery, foal lung maturation, and mare gestation. Unopened kits are shipped refrigerated; reconstituted standard aliquots should be stored at -20 °C and protected from light to limit freeze-thaw loss of antibody binding activity.