Epinephrine ELISA Kit

This competitive enzyme-linked immunosorbent assay (ELISA) from Assay Genie is designed for the quantitative measurement of epinephrine, also known as adrenaline, a vital hormone and neurotransmitter that plays a central role in the body's response to stress and danger. The kit is intended for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with reactivity reported as universal. Detection is colorimetric, performed in a competitive ELISA format coated with antigen, providing a sensitive and specific tool for studying epinephrine in research applications.

The assay offers a sensitivity of 4.688 pg/mL and a measurable range of 7.813–500 pg/mL. Linearity has been demonstrated in serum (n=10) at dilutions of 1:2 (85–104%), 1:4 (92–104%), and 1:8 (98–105%); in EDTA plasma (n=10) at 1:2 (83–98%), 1:4 (89–101%), and 1:8 (84–100%); and in heparin plasma (n=10) at 1:2 (82–100%), 1:4 (81–96%), and 1:8 (88–98%). Spike-and-recovery performance was 86–102% (average 91%) in serum (n=10), 85–101% (average 94%) in EDTA plasma (n=10), and 87–105% (average 97%) in heparin plasma (n=10). The kit is supplied sealed and should be stored at 2–8°C; it should not be cryopreserved.

The workflow begins with equilibrating the TMB substrate for 30 minutes at room temperature, after which 50 µL of standard or sample and 50 µL of biotin-labeled antibody are added to each well and incubated at 37°C for 45 minutes. The plate is washed five times with wash buffer, TMB substrate is added, and color is developed in the dark at 37°C for 10–20 minutes. After stopping the reaction, absorbance is read at 450 nm using a microplate reader. By accurately quantifying epinephrine levels, the kit supports investigation of physiological and pathological processes linked to this molecule, including heart disease, asthma, and anxiety disorders.