EPHB6 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit is designed for the qualitative measurement of EPHB6 (Ephrin Type-B Receptor 6) protein expression in cultured cells. The kit uses a colorimetric detection method read at 450 nm, with a stated dynamic range of > 5000 Cells, and is configured in a 96-Well Microplate format. Reactivity is listed for Human, Mouse, and Rat samples, and the assay is intended for adherent cells.

The workflow is lysate-free and uses an indirect ELISA format: EPHB6 is captured by target-specific primary antibodies and detected with HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition. A monoclonal anti-GAPDH antibody is included as an internal positive control to normalize target absorbance values, and a Crystal Violet whole-cell staining step is provided to determine cell density so absorbance readings can be corrected for plating differences. The kit can be used to measure relative EPHB6 levels in cultured cells and to screen the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on EPHB6 expression.

EPHB6 is described as a kinase-defective receptor for ephrin-B family ligands, binding ephrin-B1 and ephrin-B2, and modulating cell adhesion and migration, with reported effects on JNK activation, T-cell receptor-induced IL-2 secretion, and CD25 expression. The protein is catalogued at UniProt ID O15197, Gene ID 2051, and OMIM 602757, with a molecular weight of 57,498 Da. Dysregulation of EPHB6 has been implicated in cancer and neurological disorders, making it a target of research interest. Recommended storage is 4 °C with a stability of 6 Months.