EPHB1/2 (Phospho-Tyr594/604)Colorimetric Cell-Based ELISA Kit

A high-quality In-Cell ELISA Kit from Assay Genie designed to detect and quantify levels of phosphorylated EPHB1 and EPHB2 proteins in cell lysates and tissue samples. The assay uses a colorimetric detection method at 450 nm and is reactive with human, mouse, and rat samples, with a dynamic range of > 5000 cells. It is intended for adherent cells and supports a lysate-free, high-throughput workflow.

The kit employs an indirect ELISA format, in which EPHB1/2 (Phospho-Tyr594/604) is captured by specific primary antibodies and detected using HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, and the Crystal Violet whole-cell staining method may be used to determine cell density. The kit is suited to measuring the relative amounts of phosphorylated EPHB1/2 in cultured cells, and to screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on EPHB1/2 phosphorylation.

EPHB1 and EPHB2 are members of the Eph receptor tyrosine kinase family, which regulate cell signalling pathways involved in adhesion, migration, and proliferation. Phosphorylation at Tyr594/604 modulates the activity of these receptors and their downstream signalling cascades, supporting research applications in neuronal development, tumour progression, and immune response.