ELF1 Transcription Factor Activity Assay

The ELF1 Transcription Factor Activity Assay from Assay Genie enables researchers to quantify DNA-binding activity of the ETS-family transcription factor ELF1 in nuclear and cell lysates. ELF1 regulates gene expression programs in hematopoietic and immune cells, and aberrant activity has been linked to lymphoma, prostate cancer progression, and autoimmune signaling pathways. The colorimetric DNA-binding immunoassay format uses a double-stranded oligonucleotide probe immobilized on a microplate to capture active ELF1 from your sample, which is then detected with a transcription-factor-specific antibody. Each kit provides reagents for 96 individual reactions and is applicable to chromatin-binding studies, transcription factor profiling, and mechanistic gene regulation research in human, mouse, and rat samples. Store kit components refrigerated at 4 degrees C unless otherwise noted on the protocol insert to preserve antibody activity and probe integrity.