eIF4B Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed for the accurate measurement of EIF4B (Eukaryotic Translation Initiation Factor 4B) protein levels directly in cultured cells. The colorimetric detection is read at 450 nm, and the assay is reactive with human and mouse samples with a dynamic range of > 5000 Cells. EIF4B is a key protein in the regulation of protein synthesis and translation initiation, and its dysregulation has been linked to cancer and metabolic disorders, making it a valuable biomarker for studies of cellular processes and disease mechanisms.

The kit provides a convenient, lysate-free, high-throughput indirect ELISA format. EIF4B is captured by specific primary antibodies, and HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalization, and a Crystal Violet whole-cell staining method is provided to determine cell density, enabling absorbance values to be normalized to cell amounts. The assay supports measurement of relative EIF4B levels in cultured cells and screening of the effects that various treatments, inhibitors (such as siRNA or chemicals), or activators have on EIF4B. Storage is at 4°C with a stated stability of 6 Months.