EGR1/2 Transcription Factor Activity Assay

The EGR1/2 Transcription Factor Activity Assay from Assay Genie is a DNA-binding ELISA platform that quantifies the functional DNA-binding activity of Early Growth Response proteins 1 and 2 in nuclear and cell lysates. EGR1 and EGR2 are zinc-finger transcription factors of the immediate-early gene family that recognize GC-rich consensus motifs in target gene promoters, regulating cellular responses to mitogens, oxidative stress, and neuronal signaling. The microplate provided in this kit is pre-coated with double-stranded oligonucleotides containing EGR consensus binding sequences, allowing activated transcription factors from your nuclear extract to bind in a sequence-specific manner. Bound complexes are detected with anti-EGR1 and anti-EGR2 antibodies, providing a measurable readout of transcription factor activation rather than total protein abundance. Each kit provides reagents sufficient for 96 Assays and is validated for human, mouse, and rat samples, supporting studies in cancer biology, neuroscience, immunology, and signal transduction research. Nuclear extracts are typically prepared from cultured cells or fresh tissue using a suitable lysis buffer, with a recommended sample volume of approximately 10 uL per well. Store kit components at -20 degrees C, protect from light, and limit repeated freeze-thaw cycles of the antibody and oligonucleotide reagents to preserve binding activity. For research use only, this assay offers a streamlined alternative to traditional electrophoretic mobility shift assays (EMSAs) for profiling EGR pathway activation across multiple experimental conditions.